Journal: Oncology Research
Article Title: PFDN6L Gene Predicts Good Prognosis Associated with Its Inhibition of the Stem-Ness Properties in Hepatocellular Carcinoma
doi: 10.32604/or.2025.067628
Figure Lengend Snippet: PFDN6L suppressed the stem cell characteristics of HCC. ( A ) The impact of PFDN6L overexpression on the in vitro sphere formation ability of HCC stem cells was analyzed via an in vitro sphere formation assay. ( B ) qRT-PCR results showed the transcript levels of PFDN6L, CD133, CD44, and Notch1 in the control group and the PFDN6L overexpression group. ( C ) The protein expression levels of CD133, CD44, and SOX2 were measured by Western blot. Data are presented as the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Article Snippet: CD133 + PLC/PRF/5 hepatocellular carcinoma cells were magnetically isolated from PFDN6L-overexpressing and control groups using a CD133 MicroBead Kit (Miltenyi Biotec, 130-097-049, Bergisch Gladbach, Germany).
Techniques: Over Expression, In Vitro, Tube Formation Assay, Quantitative RT-PCR, Control, Expressing, Western Blot